{"product_id":"abcam-ab155639","title":"Abcam, ab155639, Recombinant human EGFR protein (Active)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human EGFR protein (Active) is a Human Fragment protein in the 25 to 645 aa range with \u0026gt;95% purity, \u0026lt; 1 EU\/µg endotoxin level and suitable for SDS-PAGE, ELISA and Functional studies.The predicted molecular weight of ab155639 protein is 70.5 kDa. - Save time and ensure accurate results - use our recombinant EGFR protein as a control - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, ELISA, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Measured by its binding ability in a functional ELISA.Immobilized Cetuximab at 2 μg\/mL (100 μL\/well) can bind ab155639 with a linear range of 0.006-0.025 μg\/mL.,\u003cbr\u003e\nAccession:P00533,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in water,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: PBS, 5% Trehalose\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our bioactive recombinant human EGFR protein ab155639 as a positive control in SDS-PAGE. The ab155639 protein migrates as 95-110 kDa under reducing condition in SDS-PAGE due to glycosylation\u003cbr\u003e\nAnalyze your ELISA data using the rEGFR ab155639 protein to generate and plot the standard curve.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nCheck out our ELISA protocol for more information\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.\u003cbr\u003e\nPathways\u003cbr\u003e\nEGFR is a central player in the MAPK and PI3K\/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.\u003cbr\u003e\nEGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843812741289,"sku":"ab155639","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab155639","provider":"Iright","version":"1.0","type":"link"}