{"product_id":"abcam-ab155901","title":"Abcam, ab155901, Creatine Kinase Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nCreatine Kinase Activity Assay Kit (Colorimetric) ab155901 is a simple and fast assay (1 h assay time) for creatine kinase (CK) activity. Readout on any colorimetric (450 nm) plate reader. - Kit includes standard curve for quantitation, and positive control enzyme - Cited in \u0026gt; 50 publications. - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:\u0026lt; 1 mU\/well,\u003cbr\u003e\nRange:2 - 10 nmol\/well,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.\u003cbr\u003e\nCreatine kinase assay protocol summary:\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reaction mix\u003cbr\u003e\n- Analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nCreatine Kinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:\u003cbr\u003e\nHuman embryonic cardiomyocyte cell line H9C2\u003cbr\u003e\nMouse and rat glomerular tissues\u003cbr\u003e\nMouse skeletal muscle samples\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Mao M et al. 2023\u003cbr\u003e\n2-Del Nogal Avila M et al. 2022\u003cbr\u003e\n3- Buchalter D et al. 2023\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.\u003cbr\u003e\nCreatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843544404137,"sku":"ab155901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab155901","provider":"Iright","version":"1.0","type":"link"}