{"product_id":"abcam-ab157747","title":"Abcam, ab157747, Anti-Myosin light chain (phospho S1) antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal MYL9 phospho S1 antibody. Suitable for WB, ICC\/IF and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Cow MYL9 conjugated to Keyhole Limpet Haemocyanin.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Cow MYL9 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q5E9E2,\u003cbr\u003e\nSpecificity:The immunogen sequence is highly conserved in Human, rat and mouse MYL9, and in other smooth muscle and non-muscle Myosin light chains.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab157747 was affinity purified using phospho-Myosin light chain (Ser-1) peptide (without carrier)., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMyosin light chain often referred to as MLC or the light chain myosin plays an important role in muscle contraction by binding to myosin heavy chains. This protein has a molecular mass of approximately 20 kDa and exists in smooth and striated muscle tissues as well as some non-muscle cells. MLC participates in regulating muscle contraction by interacting with myosin head groups during the ATPase activity which influences the actin-myosin interactions essential for muscle contraction.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMyosin light chain interaction with myosin heavy chains regulates muscle contraction and relaxation. This small protein is a component of the larger myosin II complex which functions by modulating the motor activity on actin filaments. The phosphorylation state of the myosin light chain is critical in controlling the binding affinity of myosin to actin therefore directly affecting muscle contraction and cellular movement. In smooth muscles its phosphorylation by myosin light chain kinase increases the myosin ATPase activity contributing to muscle contractility.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe action of myosin light chain fits into essential signaling processes regulating muscle function and cell motility. One significant pathway is the Rho\/Rho-kinase pathway where phosphorylation of the myosin light chain modulates the contraction of smooth muscle and influences cell shape changes. Additionally the calcium-Calmodulin-Myosin Light Chain Kinase pathway also affects its phosphorylation status impacting smooth muscle contraction. Proteins such as phosphomyosin and myosin phosphatase interact within these pathways reinforcing the functional versatility of myosin light chain.\u003cbr\u003e\nMyosin light chain is linked to conditions such as asthma and hypertension. Altered phosphorylation levels of this protein can cause increased smooth muscle contraction which is a contributing factor in the pathogenesis of these diseases. Asthma involves hyperresponsiveness of airway smooth muscle while hypertension often features increased vascular smooth muscle tone. The protein is functionally connected to tension-generating mechanisms involving myosin phosphatase providing a therapeutic target in managing muscle tone abnormalities in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854068175017,"sku":"ab157747","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab157747","provider":"Iright","version":"1.0","type":"link"}