{"product_id":"abcam-ab158333","title":"Abcam, ab158333, Recombinant Human Endothelin 1 protein","description":"\u003cp\u003eSize: 10µg\u003cbr\u003e\nRecombinant Human Endothelin 1 protein is a Human Fragment protein, in the 113 to 212 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.\u003cbr\u003e\nKey facts\u003cbr\u003e\nExpression system:Wheat germ,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:ELISA, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P05305,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProtein concentration is lot specific and is above or equal to 0.07 ug\/uL.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEndothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.\u003cbr\u003e\nET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843862515881,"sku":"ab158333","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab158333","provider":"Iright","version":"1.0","type":"link"}