{"product_id":"abcam-ab168341","title":"Abcam, ab168341, Anti-Galactosidase alpha antibody [EP5828(2)]","description":"\u003cp\u003eSize: 20µL \/ 10µL-TRIAL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Galactosidase alpha antibody [EP5828(2)] (ab168341) is a rabbit monoclonal antibody detecting Galactosidase alpha in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP5828(2),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, IP, ICC\/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Galactosidase alpha antibody [EP5828(2)] (ab168341) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of Galactosidase alpha?\u003cbr\u003e\nAnti-Galactosidase alpha [EP5828(2)] (ab168341) specifically detects a band for Galactosidase alpha (UniProt: P06280) at a molecular weight of 49kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Galactosidase alpha [EP5828(2)] (ab168341) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-Galactosidase alpha antibody [EP5828(2)] (ab168341) has been confirmed by Western blot testing in GLA Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EP5828(2)] also available for your convenience: ab168341, Carrier free -\u003cbr\u003e\nab215258\u003cbr\u003e\n, APC -\u003cbr\u003e\nab310830\u003cbr\u003e\n, PE -\u003cbr\u003e\nab310906\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab310964\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab311082\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab311676\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312951\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab313160\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321155\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha-galactosidase also known as alpha-D-galactosidase or A-galactosidase is an enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It has a molecular mass of approximately 50000 Daltons. This enzyme is expressed in various tissues including the liver heart and kidneys. It functions by efficiently removing alpha-galactose residues from target molecules a process important for maintaining cellular function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of alpha-galactosidase involves the breakdown of complex carbohydrates particularly those containing alpha-galactosidic bonds. It does not operate as part of a complex but rather functions independently to cleave these specific bonds in glycolipids and glycoproteins. This activity is critical for degrading langerin and other gangliosides preventing the accumulation of these molecules in the lysosomes. By continuously performing this function alpha-galactosidase maintains glycosphingolipid homeostasis ensuring efficient cellular metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of alpha-galactosidase in metabolic and lysosomal catabolic pathways is significant. One critical pathway is the glycolipid metabolism pathway where it acts alongside related enzymes such as beta-galactosidase. It also participates in the lysosomal degradation pathway working in conjunction with other lysosomal enzymes to prevent substrate buildup. The interaction with enzymes like lysosomal hydrolases ensures the proper breakdown of complex molecules into simpler ones that cells can utilize or excrete.\u003cbr\u003e\nDefects in the alpha-galactosidase enzyme lead to the lysosomal storage disorder known as Fabry disease. This genetic disorder causes the accumulation of globotriaosylceramide due to deficient alpha-galactosidase activity. Another disorder associated with malfunctioning alpha-galactosidase is cardiac complications due to tissue storage of glycolipids. In both cases the lack of enzyme activity disrupts cellular and tissue functions linking alpha-galactosidase to clinical symptoms seen in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844058796201,"sku":"ab168341","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab168341","provider":"Iright","version":"1.0","type":"link"}