{"product_id":"abcam-ab171449","title":"Abcam, ab171449, Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-CPT1A antibody [8F6AE9] - Alexa Fluor® 488 conjugated (ab171449) is a mouse monoclonal antibody detecting CPT1A in  Flow Cytometry (Intra), Flow Cytometry, ICC\/IF . Suitable for  Human . - Clone 8F6AE9 is the most cited clone to CPT1A - KO validated for confirmed specificity - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:8F6AE9,\u003cbr\u003e\nIsotype:IgG2b,\u003cbr\u003e\nConjugation:Alexa Fluor® 488,\u003cbr\u003e\nExcitation\/Emission:Ex: 495nm, Em: 519nm,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAlexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-CPT1A [8F6AE9] Alexa Fluor® 488 (ab171449) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nRelated products\u003cbr\u003e\nAntibody clone 8F6AE9 is also available pre-conjugated to a variety of labels for your convenience – Anti-CPT1A Alexa Fluor® 488 [8F6AE9] (ab171449).\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449) has been confirmed by Flow Cytometry testing in CPT1A Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [8F6AE9] also available for your convenience:\u003cbr\u003e\nab128568\u003cbr\u003e\n, Alexa Fluor® 488 - ab171449, HRP -\u003cbr\u003e\nab198494\u003cbr\u003e\n, Carrier free - ab300265\u003cbr\u003e\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Precipitation Ammonium Sulphate, Purification notes-Purity is near homogeneity as judged by SDS-PAGE. ab171449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation., Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze \/ thaw cycle, Stable for 12 months at -20°C, Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.\u003cbr\u003e\nPathways\u003cbr\u003e\nCPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.\u003cbr\u003e\nCPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848575275177,"sku":"ab171449","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab171449","provider":"Iright","version":"1.0","type":"link"}