{"product_id":"abcam-ab174717","title":"Abcam, ab174717, Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nMouse Monoclonal LAMA3 antibody. Carrier free. Suitable for IHC-Fr and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:P3H9-2,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab174717 is the carrier-free version of\u003cbr\u003e\nab78286\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLaminin 5 also known as laminin-332 is a heterotrimeric glycoprotein composed of alpha-3 beta-3 and gamma-2 chains with a molecular mass of about 460 kDa. Laminin 5 is expressed in the basement membrane zone of epithelial tissues playing a significant role in tissue structure. This molecule is widely present in areas such as skin lungs and other epithelial cell surfaces facilitating cellular functions like adhesion migration and differentiation. Researchers often use laminin antibodies including those for laminin IHC to visualize this molecule in various tissue samples.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLaminin 5 forms part of the integrin complexes that modulate interactions between cells and the extracellular matrix. It provides critical structural support and influences cell behavior within the tissue microenvironment. Laminin 5 participates in the anchoring of keratinocytes to the underlying basement membrane which is essential for skin integrity and maintenance. Its interaction with laminin receptors on the cell surface strengthens the cellular attachment impacting the overall integrity and mechanical resistance of tissues.\u003cbr\u003e\nPathways\u003cbr\u003e\nLaminin 5 is integral to the hemidesmosome assembly pathway and the MAPK\/ERK signaling pathway. These pathways involve its interactions with integrins such as alpha-6 beta-4 and other proteins like dystroglycan. Laminin molecules including laminin 5 influence the downstream signaling of MAPK\/ERK which governs processes like cellular proliferation and differentiation. The linkage with integrin-mediated signaling pathways allows laminin 5 to participate actively in cell adhesion mechanisms and responses to extracellular matrix changes.\u003cbr\u003e\nLaminin 5 associates with junctional epidermolysis bullosa and cancer progression. Mutations in the genes encoding laminin 5 subunits lead to junctional epidermolysis bullosa a disorder marked by fragile skin and blister formations due to defective basement membrane anchoring. In cancer altered expression of laminin 5 facilitates tumor invasion and metastasis often involving interactions with matrix metalloproteinases (MMPs) and other extracellular matrix components. These connections underline the role of laminin 5 in pathological processes highlighting its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855366410409,"sku":"ab174717","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab174717","provider":"Iright","version":"1.0","type":"link"}