{"product_id":"abcam-ab176646","title":"Abcam, ab176646, JNK 1\/2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nJNK 1\/2 ELISA Kit is a single-wash 90-min Simplestep used to quantify JNK 1\/2 with a sensitivity of 0.1 ng\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Homogenate, Cell Lysate,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nSensitivity:= 0.1 ng\/mL,\u003cbr\u003e\nRange:0.2 - 20 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's JNK1\/2\u003cbr\u003e\nin vitro\u003cbr\u003e\nSimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of JNK1\/2 protein in Human and mouse cells.\u003cbr\u003e\nThe SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody\/analyte\/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nJNK 1\/2 also known as c-Jun N-terminal kinases represents a group of kinases with a molecular mass around 46-55 kDa. These kinases are ubiquitously expressed across various tissue types and play an essential role in cellular signaling. JNK 1\/2 phosphorylates transcription factors such as c-Jun which modulates gene expression. The target is essential for transducing extracellular signals to the nucleus influencing various physiological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nJNK 1\/2 takes part in important cellular activities including apoptosis differentiation and inflammation. The kinases form part of the MAPK (mitogen-activated protein kinase) signaling pathway and do not function alone but rather as components of larger signaling complexes. JNK 1\/2 interacts with scaffold proteins like JNK-interacting protein 1 (JIP1) which helps in specifying their signaling outcomes. This interaction impacts numerous biological functions showcasing the versatility of JNK 1\/2 in cellular dynamics.\u003cbr\u003e\nPathways\u003cbr\u003e\nJNK 1\/2 integrates into the MAPK and stress-activated protein kinase pathways. These pathways mediate responses to stress stimuli such as cytokines ultraviolet irradiation and heat shock. JNK 1\/2 relates to other proteins including the upstream kinases MKK4 and MKK7 which phosphorylate and activate JNK 1\/2. This signaling module ultimately affects downstream targets like transcription factor c-Jun contributing to cellular responses.\u003cbr\u003e\nJNK 1\/2 features prominently in conditions such as cancer and diabetes. In cancer dysregulation of JNK pathways can lead to unchecked cell proliferation and survival. The interplay between JNK 1\/2 and proteins like tumor suppressor p53 highlights its role in controlling cell death. In diabetes JNK 1\/2 influences insulin resistance linking its activity to metabolic pathways and proteins like insulin receptor substrates. Dysregulated JNK activity becomes a critical factor in the pathophysiology of these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855615414441,"sku":"ab176646","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab176646","provider":"Iright","version":"1.0","type":"link"}