{"product_id":"abcam-ab178013","title":"Abcam, ab178013, Human IL-6 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman IL-6 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-6 (IL-6) with a sensitivity of 1.6 pg\/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450nm readout; works on any plate reader - Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput - Broad sample compatibility – including CSF - Cited in over 210 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma, Cerebral Spinal Fluid,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.6 pg\/mL,\u003cbr\u003e\nRange:7.8 - 500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-6 ELISA Kit ab178013 is a rapid single-wash 90-min ELISA kit to measure Human IL-6 in cell culture supernatant, serum, plasma, cerebral spinal fluid. This SimpleStep ELISA® sensitivity is 1.6 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-6 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit. Recombinant Human IL-6Ra was prepared at 50 ng\/mL and 500 pg\/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant Human IL-6Ra was prepared at 50 ng\/mL and 500 pg\/mL and tested for interference. No interference with was observed.\u003cbr\u003e\nHuman IL-6 ELISA Kit ab178013 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human IL-6 ELISA kit ab178013\u003cbr\u003e\nHuman IL-6 ELISA kit ab178013 has been used to assess inflammation in different physio-pathological contexts such as obesity\u003cbr\u003e\n, coronary artery disease\u003cbr\u003e\n, and rheumatoid arthritis\u003cbr\u003e\nReferences:\u003cbr\u003e\nShaohong Yu et al. 2023, PMID: 38156278,\u003cbr\u003e\nYongshun W. et al. 2023, PMID:38054817,\u003cbr\u003e\nYaju Tan et al. 2024, PMID: 37997635\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human IL-6 ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human\/Monkey IL-6 Antibody Pair - BSA and Azide free\u003cbr\u003e\nab243973\u003cbr\u003e\n- Anti-IL-6 antibody [IL-6-10] - BSA and Azide free (Capture)\u003cbr\u003e\nab244758\u003cbr\u003e\n- Anti-IL-6 antibody [IL-6-5] - BSA and Azide free (Detector)\u003cbr\u003e\nab244886\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman IL-6 ELISA kit ab178013 is commonly used to measure IL-6 as a marker of inflammation.\u003cbr\u003e\nOther ELISA kits to measure inflammatory markers include:\u003cbr\u003e\n- Human TNF alpha ELISA kit\u003cbr\u003e\nab181421\u003cbr\u003e\n- Human IL-8 ELISA kit\u003cbr\u003e\nab214030\u003cbr\u003e\n- Human IL-1 beta ELISA kit\u003cbr\u003e\nab214025\u003cbr\u003e\nIn glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.\u003cbr\u003e\nExplore our comprehensive range of glioblastoma research solutions here: https:\/\/www.abcam.com\/en-us\/products\/product-recommendations\/glioblastoma-research-tools\u003cbr\u003e\nIn Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.\u003cbr\u003e\nExplore our extensive Alzheimer's research solutions here: https:\/\/www.abcam.com\/en-us\/products\/product-recommendations\/alzheimers-disease-tools\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843535065257,"sku":"ab178013","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab178013","provider":"Iright","version":"1.0","type":"link"}