{"product_id":"abcam-ab183322","title":"Abcam, ab183322, Anti-CD14 antibody [SP192]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 500µL \/ 1mL\u003cbr\u003e\nAnti-CD14 antibody [SP192] (ab183322) is a rabbit monoclonal antibody detecting CD14 in  Western Blot, Flow Cytometry, IHC-P . Suitable for  Human . - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:SP192,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow Cyt, WB, IHC-P, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-CD14 antibody [SP192] (ab183322) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of CD14?\u003cbr\u003e\nAnti-CD14 [SP192] (ab183322) specifically detects a band for CD14 (UniProt: P08571) at a molecular weight of 40kDa.\u003cbr\u003e\nCollaboration\u003cbr\u003e\nAnti-CD14 [SP192] (ab183322) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-CD14 [SP192] (ab183322) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-CD14 [SP192] (ab183322) has over 5 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [SP192] also available for your convenience: ab183322, Carrier free -\u003cbr\u003e\nab230903\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab302698\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab302826\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab305119\u003cbr\u003e\n, PE -\u003cbr\u003e\nab307635\u003cbr\u003e\n, APC -\u003cbr\u003e\nab314412\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nShipping conditions update: ambient shipping\u003cbr\u003e\nThis product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.\u003cbr\u003e\nWhy the change?\u003cbr\u003e\nIt’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.\u003cbr\u003e\nWhat you need to know\u003cbr\u003e\nAmbient shipments come clearly marked on the delivery note.\u003cbr\u003e\nNo ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.\u003cbr\u003e\nWarranty coverage remains fully valid, aligned with our validated shipping method.\u003cbr\u003e\nPlease store the product as per the datasheet instructions upon receipt.\u003cbr\u003e\nFind out more -\u003cbr\u003e\nhttps:\/\/www.abcam.com\/en-us\/support\/shipping-storage-support\/ambient-shipping\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A\/G, Purification notes-Purified from TCS by protein A\/G., Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.\u003cbr\u003e\nResearchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844073279657,"sku":"ab183322","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab183322","provider":"Iright","version":"1.0","type":"link"}