{"product_id":"abcam-ab183360","title":"Abcam, ab183360, Human FAS ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman FAS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FAS with a sensitivity of 26 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 26 pg\/mL,\u003cbr\u003e\nRange:46.88 - 3000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAs of October 15, 2019, Human FAS ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.\u003cbr\u003e\nHuman FAS ELISA kit (ab183360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAS protein in human cell culture supernatant, serum, and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human FAS with 2 pg\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.\u003cbr\u003e\nFAS is a plasma membrane receptor for FASLG ligand (TNFSF6\/FASLG). Upon ligand binding, the FASLG Receptor is activated and the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) activation mediating apoptosis. FASLG-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 of the FASLG Receptor block apoptosis as they sequester the Fas ligand.\u003cbr\u003e\nThis kit uses a recombinant protein fragment corresponding to the extracellular portion (amino acid 17-172) of the full length Human Fas Receptor protein as standard sample.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843584905385,"sku":"ab183360","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab183360","provider":"Iright","version":"1.0","type":"link"}