{"product_id":"abcam-ab186027","title":"Abcam, ab186027, Cellular ROS Assay Kit (Red)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nCellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in cell cultures. - Add ROS sensor reagent to cell cultures, treat cells to induce ROS production, and readout on a fluorometric (Ex\/Em 520\/605 nm) plate reader. - Cited in \u0026gt;50 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based (quantitative),\u003cbr\u003e\nAssay time:60m,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluorescence microscope\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells. The red dye used in the ROS assay protocol is cell-permeable and generates red fluorescence when it reacts with ROS. The kit is an optimized \"mix and read\" assay format that is compatible with HTS liquid handling instruments.\u003cbr\u003e\nab186027 provides an ultrasensitive fluorometric one-step ROS assay. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex\/Em = 520\/605 nm and can be used to either quantify ROS levels in cells or to screen ROS inhibitors.\u003cbr\u003e\nReactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.\u003cbr\u003e\nRelated ROS assay kits\u003cbr\u003e\nTo measure reactive oxygen species within cells, we recommend DCFDA assay kit\u003cbr\u003e\nab113851\u003cbr\u003e\n. Alternative ROS assays are available in orange (\u003cbr\u003e\nab186028\u003cbr\u003e\n), red (ab186027), and deep red (\u003cbr\u003e\nab186029\u003cbr\u003e\n). DCF ROS\/RNS Assay Kit\u003cbr\u003e\nab238535\u003cbr\u003e\nis used to measure ROS in biofluids, culture supernatants and cell lysates.\u003cbr\u003e\nFor assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use\u003cbr\u003e\nab139476\u003cbr\u003e\n; to assay ROS, superoxides, and reactive nitrogen species use\u003cbr\u003e\nab139473\u003cbr\u003e\n; to assay superoxides use\u003cbr\u003e\nab219943\u003cbr\u003e\nOther notes\u003cbr\u003e\nPreviously called Cellular Reactive Oxygen Species Detection Assay Kit (Red Fluorescence).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843598733481,"sku":"ab186027","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab186027","provider":"Iright","version":"1.0","type":"link"}