{"product_id":"abcam-ab186029","title":"Abcam, ab186029, Cellular ROS Assay Kit (Deep Red)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nCellular ROS Assay Kit (Deep Red) ab186029 uses a Reactive oxygen species (ROS) sensor to quantify ROS in live cells. - Sensitive one-step fluorimetric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation - Suitable for microplate reader and fluorescence microscope - Flexible formats: use with 96-well or 384-well microtiter plate - Cited in over 55 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCellular ROS Assay Kit (Deep Red) ab186029 uses an ROS sensor to quantify ROS in live cells. The ROS Deep Red dye is cell-permeable and generates Deep Red fluorescence when it reacts with ROS. The fluorescence signal of the ROS Deep Red Dye can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry.\u003cbr\u003e\nab186029 provides a sensitive fluorometric, one-step assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader at Ex\/Em = 650\/675 nm or a fluorescent microscope with with Cy5 filter.\u003cbr\u003e\nPreviously called Cellular Reactive Oxygen Species Detection Assay Kit (Deep Red Fluorescence). Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. **Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader. To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (\u003cbr\u003e\nab186028\u003cbr\u003e\n), red (\u003cbr\u003e\nab186027\u003cbr\u003e\n), and deep red (ab186029).\u003cbr\u003e\nab238535\u003cbr\u003e\nis used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use\u003cbr\u003e\nab139476\u003cbr\u003e\n; to assay ROS, superoxides, and reactive nitrogen species use\u003cbr\u003e\nab139473\u003cbr\u003e\n; to assay superoxides use\u003cbr\u003e\nab219943\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843599945897,"sku":"ab186029","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab186029","provider":"Iright","version":"1.0","type":"link"}