{"product_id":"abcam-ab188029","title":"Abcam, ab188029, Anti-MIRO1 antibody [CL1083]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-MIRO1 antibody [CL1083] (ab188029) is a mouse monoclonal antibody detecting MIRO1 in  Western Blot, IHC-P . Suitable for  Human . - KO validated for confirmed specificity\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:CL1083,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human RHOT1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.Q8IXI2\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-MIRO1 antibody [CL1083] (ab188029) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of MIRO1?\u003cbr\u003e\nAnti-MIRO1 [CL1083] (ab188029) specifically detects a band for MIRO1 (UniProt: Q8IXI2) at a molecular weight of 71kDa.\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-MIRO1 antibody [CL1083] (ab188029) has been confirmed by Western blot testing in RHOT1 Knockout HeLa cell line,\u003cbr\u003e\nab265792\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1\/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nMIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.\u003cbr\u003e\nMIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845034201257,"sku":"ab188029","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab188029","provider":"Iright","version":"1.0","type":"link"}