{"product_id":"abcam-ab189579","title":"Abcam, ab189579, Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin (SERPINA1) with a sensitivity of 13 ng\/mL. Suitable to work with Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput. - Cited in over 10 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 13 ng\/mL,\u003cbr\u003e\nRange:31.25 - 2000 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is a rapid single-wash 90-min ELISA kit to measure Human alpha 1 Antitrypsin in serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman alpha 1 Antitrypsin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nHuman IgM, human IgA and human IgE were prepared at 10 ng\/mL and 250 ng\/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng\/mL and only 0.4% cross-reactivity at 250 ng\/mL of IgA.\u003cbr\u003e\nPurified human IgG was assayed at 5 ng\/mL in the presence and absence of 250 ng\/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.\u003cbr\u003e\nHuman alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers have used Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579\u003cbr\u003e\nHuman alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 has been used in publications to study bioengineered liver grafts for treating acute liver failure\u003cbr\u003e\n, alpha 1 antitrypsin for predicting acute kidney injury after cardiopulmonary bypass\u003cbr\u003e\n, and cystatin C correlation with alpha-1 antitrypsin in asthma\u003cbr\u003e\nReferences:\u003cbr\u003e\nJeff J Ross et al. 2022 PMID: 37892931,\u003cbr\u003e\nJun Ai et al. 2019 PMID: 32030221,\u003cbr\u003e\nMohamad Sadikin et al. 2023 PMID: 38094096\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) only requires a maxmum of 12.5µL\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is commonly used to study liver function and inflammation.\u003cbr\u003e\nOther kits for measuring liver function and inflammation include:\u003cbr\u003e\n- Human Albumin ELISA kit\u003cbr\u003e\nab179887\u003cbr\u003e\n- Human ALT ELISA kit\u003cbr\u003e\nab234578\u003cbr\u003e\n- Human AST ELISA Kit\u003cbr\u003e\nab263881\u003cbr\u003e\nalpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843618623657,"sku":"ab189579","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab189579","provider":"Iright","version":"1.0","type":"link"}