{"product_id":"abcam-ab191468","title":"Abcam, ab191468, Anti-CRISPR-Cas9 antibody [7A9-3A3]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-CRISPR-Cas9 antibody [7A9-3A3] (ab191468) is a mouse monoclonal antibody detecting CRISPR-Cas9 in  Western Blot, ICC\/IF . - Over 50 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:7A9-3A3,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Ab191468 detects Cas9 and dCas9. This product has not been tested against SaCas9. Please contact Abcam Scientific Support for more information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-CRISPR-Cas9 antibody [7A9-3A3] (ab191468) is a House Mouse Monoclonal antibody and is validated for use in ICC\/IF, WB in human, mouse, pig, rabbit, rat samples.\u003cbr\u003e\nAnti-CRISPR-Cas9 antibody [7A9-3A3] (ab191468) has been cited over 55 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcams high quality validation processes ensure Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab191468) has high sensitivity and specificity.\u003cbr\u003e\nAnti-CRISPR-Cas9 antibody [7A9-3A3] (ab191468) has 11 independent reviews from customers.\u003cbr\u003e\nAnti-CRISPR-Cas9 antibody [7A9-3A3] (ab191468) specifically detects CRISPR-Cas9 (UniProt ID: Q99ZW2; Molecular weight: 158kDa) and is sold in 100 ug selling sizes.\u003cbr\u003e\nAntibody clone 7A9-3A3 is also available pre-conjugated to a variety of labels for your convenience - HRP (ab2258).\u003cbr\u003e\nCRISPR-Cas9 technology enables precise gene editing to enhance cancer immunotherapy. It allows for the modification of immune cells to better target and destroy cancer cells, improving the efficacy of treatments like CAR-T cell therapy. Additionally, CRISPR-Cas9 can be used to identify and target immune checkpoint molecules, overcoming immune evasion by tumors.\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.\u003cbr\u003e\nPathways\u003cbr\u003e\nCRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70\/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.\u003cbr\u003e\nCRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845023977641,"sku":"ab191468","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab191468","provider":"Iright","version":"1.0","type":"link"}