{"product_id":"abcam-ab193267","title":"Abcam, ab193267, Glutathione Sepharose®","description":"\u003cp\u003eSize: 1mL\u003cbr\u003e\nGlutathione Sepharose® (ab193267) is part of the reagents, controls \u0026amp; accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:PurificationSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 80% PBS, 20% Ethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nContents:\u003cbr\u003e\nSupplied as a 50 % slurry in 20 % Ethanol \/ PBS; \u0026gt; 3 mg (10 μmol) glutathione per mL Sepharose\u003cbr\u003e\nbeads.\u003cbr\u003e\nFeatures:\u003cbr\u003e\nThe Glutathione-S-Transferase (GST) gene fusion system has been widely used for the overexpression of foreign genes in\u003cbr\u003e\nE. Coli\u003cbr\u003e\nand other expression systems. The GST-tagged protein can be easily purified from cell lysate or media by affinity chromatography using glutathione Sepharose\u003cbr\u003e\nbeads. Abcam's Glutathione Sepharose\u003cbr\u003e\nis designed for the purification of GST fusion proteins and other glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.\u003cbr\u003e\nThese beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x\u003cbr\u003e\nwhen centrifuging.\u003cbr\u003e\nStore beads at 4°C.\u003cbr\u003e\nThe beads may be damaged above 40°C.\u003cbr\u003e\nDO NOT FREEZE.\u003cbr\u003e\nWash beads 3 times with 3x bead volume of desired buffer before use.\u003cbr\u003e\nApplications:\u003cbr\u003e\nPurification of GST-fusion proteins or other glutathione-binding proteins.\u003cbr\u003e\nSepharose is a registered trademark of GE Healthcare\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called 6555 Glutathione Sepharose. 6555-10 is the same size as the 10 ml size of ab193267.\u003cbr\u003e\nGlutathione Sepharose\u003cbr\u003e\nis prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked Sepharose\u003cbr\u003e\nbeads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity, which could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847578013865,"sku":"ab193267","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab193267","provider":"Iright","version":"1.0","type":"link"}