{"product_id":"abcam-ab194108","title":"Abcam, ab194108, Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker","description":"Size: 100µL\nAnti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 647 conjugated (ab194108) is a rabbit recombinant monoclonal antibody detecting Lamin B1 in  ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency -  Recommended for methanol-fixed samples in ICC only. Use ab229025 for compatibility with both methanol and PFA.\nKey facts\nHost species:Rabbit,\nClonality:Monoclonal,\nClone number:EPR8985(B),\nIsotype:IgG,\nConjugation:Alexa Fluor® 647,\nExcitation\/Emission:Ex: 650nm, Em: 665nm,\nCarrier free:No,\nReacts with:Human,\nApplications:ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\nSpecificity:From Jan 2026, QC testing of replenishment batches of this primary conjugate changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, the replenishment lots are subjected to bioanalytical testing only. For more information on a specific batch, please contact our Scientific Support who will be happy to help.\n\nProduct details:\nWhat is this antibody validated in?\nAlexa Fluor\n647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\nRelated products\nAntibody clone EPR8985(B) is also available pre-conjugated to a variety of labels for your convenience – Anti-Lamin B1 Alexa Fluor\n647 [EPR8985(B)] - Nuclear Envelope Marker (ab194108).\nSpecificity confirmed\nThe specificity of Alexa Fluor\n647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108) has been confirmed by Immunocytochemistry testing in LMNB1 Knockout HAP1 cells.\nOther related products\nWe have a range of other formats of antibody clone [EPR8985(B)] also available for your convenience:\nab133741\n, Alexa Fluor\nab194106\n, Alexa Fluor® 647 - ab194108, HRP -\nab194109\n, Alexa Fluor\nab216723\n, Carrier free -\nab220797\nPatented technology\nOur RabMAb\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\nRabMAb® patents\nWhat are the advantages of a recombinant monoclonal antibody?\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\n- High batch-to-batch consistency and reproducibility\n- Improved sensitivity and specificity\n- Long-term security of supply\n- Animal-free batch production\nFor more information, read more on\nrecombinant antibodies\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\n\nProperties and Storage Information:\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle, Store in the dark\n\nSupplementary Information:\nThis supplementary information is collated from multiple sources and compiled automatically.\nLamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.\nBiological function summary\nLamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.\nPathways\nLamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A\/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.\nMutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A\/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.\n","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852637982889,"sku":"ab194108","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab194108","provider":"Iright","version":"1.0","type":"link"}