{"product_id":"abcam-ab196994","title":"Abcam, ab196994, Aldolase Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nAldolase Activity Assay ab196994, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue, Suspension cells, Serum, Adherent cells,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:\u0026lt; 100 µU,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAldolase Activity Assay (Colorimetric) (ab196994), aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.\u003cbr\u003e\nThis assay kit is simple, sensitive and high-throughput adaptable and it can be used to measure aldolase activity in various cells and tissues as well as to analyze glycolysis and glucogenesis pathways.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K665 Biovision Aldolase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nAldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate \u0026amp; dihydroxyacetone.\u003cbr\u003e\nThere are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy \u0026amp; hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase.\u003cbr\u003e\nAccurate detection of aldolase activity is valuable for diagnostic and mechanistic studies.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAldolase also known as fructose-bisphosphate aldolase is an enzyme that plays a critical role in glycolysis catalyzing the reversible cleavage of fructose 16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Aldolase is a homotetramer with a molecular mass of approximately 158 kDa. It is highly expressed in liver muscle and brain tissues. Aldolase consists of three isoforms: aldolase A B and C each predominant in different tissues contributing to tissue-specific roles within the organism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAldolase catalyzes an important reaction in energy metabolism ensuring the continuation of glycolytic flux which is essential for ATP production. Aldolases do not form part of larger protein complexes. However they interact with other enzymes within the glycolytic pathway to facilitate a smooth metabolic flow. Moreover aldolase A plays an additional role in gluconeogenesis the pathway involved in synthesizing glucose from non-carbohydrate sources.\u003cbr\u003e\nPathways\u003cbr\u003e\nAldolase is an essential part of glycolysis and gluconeogenesis pathways. In glycolysis it partners with enzymes like phosphofructokinase and enolase to breakdown glucose for energy production. During gluconeogenesis aldolase works with enzymes including fructose-16-bisphosphatase to create glucose vital for maintaining blood sugar levels during fasting. Through these pathways aldolase ensures balance between energy-producing and energy-consuming states within the cell.\u003cbr\u003e\nMutations or deficiencies in aldolase particularly aldolase A have been linked to glycogen storage disease type XII which results in muscle weakness and exercise intolerance. Aldolase B deficiency is known to cause hereditary fructose intolerance leading to liver and renal complications. These conditions highlight the importance of aldolase in maintaining metabolic health with implications for proteins within the same pathways potentially affecting their functions and expression levels.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843617116329,"sku":"ab196994","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab196994","provider":"Iright","version":"1.0","type":"link"}