{"product_id":"abcam-ab204718","title":"Abcam, ab204718, Succinate Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nSuccinate Assay Kit (Colorimetric) (ab204718) provides a simple and fast method to quantify succinate levels. Readout on any colorimetric (450 nm) plate reader. - Cited in over 40 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue, Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:= 40 µM,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn Succinate Assay Kit protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm.\u003cbr\u003e\nSuccinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).\u003cbr\u003e\nThis kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.\u003cbr\u003e\nSuccinate assay protocol summary:\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reaction mix and incubate for 30 min\u003cbr\u003e\n- Analyze with a microplate reader\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nSuccinate Assay Kit (Colorimetric)has been used in a variety of sample type including:\u003cbr\u003e\n- Mouse brain\u003cbr\u003e\n- IPSC-derived cardiomyocytes\u003cbr\u003e\n- Human melanoma (C8161) cells\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Lancaster M et al. 2023\u003cbr\u003e\n2-Srivastava S et al. 2023\u003cbr\u003e\n3-Shen L et al. 2023\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.\u003cbr\u003e\nSuccinic acid (C\u003cbr\u003e\n) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C\u003cbr\u003e\n) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.\u003cbr\u003e\nThis kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSuccinic acid also known as butanedioic acid plays a significant mechanical role in cellular metabolism. It has a molecular mass of 118.09 g\/mol and is important for both prokaryotic and eukaryotic cells. This compound is expressed in mitochondria where it acts as an intermediary in critical metabolic processes. The widespread presence across different types of organisms highlights its essential role in cellular energy production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSuccinic acid contributes to energy production by participating in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. It is part of this complex cycle that produces ATP the energy currency of the cell. Succinic acid links different metabolic pathways facilitating the oxidation of nutrients to release energy. This has a broad impact on cellular function and organismal energy homeostasis emphasizing its indispensable role in general metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nSuccinic acid is heavily involved in the TCA cycle and the electron transport chain. During the TCA cycle it transforms into fumarate through succinate dehydrogenase a vital process for energy extraction in cells. Succinic acid influences the electron transport chain where it facilitates ATP synthesis by interacting with complexes like Coenzyme Q10. These pathways are central to metabolic activity demonstrating how succinic acid integrates into the broader network of cellular respiration.\u003cbr\u003e\nSuccinic acid shows connections with metabolic disorders such as mitochondrial diseases and some cancers. Abnormal accumulation or defective metabolism of succinic acid can lead to mitochondrial dysfunction contributing to disorders like Leigh's syndrome. Additionally its involvement with the succinate dehydrogenase complex indicates potential links to various cancers given how mutations in this protein complex can lead to unregulated cell growth. These connections reflect the importance of succinic acid in maintaining metabolic stability highlighting its potential as a biomarker in disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843541422249,"sku":"ab204718","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab204718","provider":"Iright","version":"1.0","type":"link"}