{"product_id":"abcam-ab205","title":"Abcam, ab205, Anti-F-actin antibody [NH3]","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nAnti-F-actin antibody [NH3] (ab205) is a mouse monoclonal antibody detecting F-actin in  Western Blot, Flow Cytometry, IHC-Fr, ICC\/IF, ELISA . Suitable for  Human, Mouse, Rabbit, Rat . - Over 120 publications - Trusted since 1999\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:NH3,\u003cbr\u003e\nIsotype:IgM,\u003cbr\u003e\nLight chain type:unknown,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Rabbit, Human, Mouse,\u003cbr\u003e\nApplications:IHC-R, WB, IHC-Fr, ICC\/IF, Flow Cyt, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nEpitope:This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.,\u003cbr\u003e\nSpecificity:Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-F-actin antibody [NH3] (ab205) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, ICC\/IF, IHC-Fr, IHC-R, IP, WB in human, mouse, rabbit, rat samples.\u003cbr\u003e\nAnti-F-actin antibody [NH3] (ab205) specifically detects F-actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 250 µg selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-F-actin antibody [NH3] (ab205) has high sensitivity and specificity.\u003cbr\u003e\nAnti-F-actin antibody [NH3] (ab205) has been cited over 126 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-F-actin antibody [NH3] (ab205) has 11 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-IgM fraction, Purification notes-Purified IgM prepared from tissue culture supernatant., Storage buffer-Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nF-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.\u003cbr\u003e\nPathways\u003cbr\u003e\nF-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.\u003cbr\u003e\nAbnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855689830569,"sku":"ab205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab205","provider":"Iright","version":"1.0","type":"link"}