{"product_id":"abcam-ab209422","title":"Abcam, ab209422, Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nAnti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422) is a rabbit recombinant monoclonal antibody in a PBS only buffer for easy conjugation. Suitable for  Western Blot, IHC-P, IHC-FrFl, ELISA, Dot Blot  in  Human, Mouse, Rat . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP1536Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IHC-P, ELISA, Dot, WB, IHC-FrFlSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.Mouse and rat species are recommended based on WB results, we do not guarantee  IHC-P for mouse and rat.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA, Dot Blot in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Alpha-synuclein?\u003cbr\u003e\nAnti-Alpha-synuclein (phospho S129) [EP1536Y] - BSA and Azide free (ab209422) specifically detects a band for Alpha-synuclein (UniProt: P37840) at a molecular weight of 14kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Alpha-synuclein (phospho S129) [EP1536Y] - BSA and Azide free (ab209422) was first used in a scientific publication in 2016 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EP1536Y] also available for your convenience:\u003cbr\u003e\nab51253\u003cbr\u003e\n, Carrier free - ab209422, Alexa Fluor® 594 -\u003cbr\u003e\nab282652\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab307166\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab310949\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab311067\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab313137\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321645\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nRat: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nSynaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.\u003cbr\u003e\nAlterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844137373865,"sku":"ab209422","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab209422","provider":"Iright","version":"1.0","type":"link"}