{"product_id":"abcam-ab210603","title":"Abcam, ab210603, Nitrotyrosine ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nNitrotyrosine ELISA Kit is a Competitive ELISA for the measurement of Nitrotyrosine in in Biofluids, Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Serum, Other biological fluids, Cell Lysate,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nSensitivity:= 50 nM,\u003cbr\u003e\nRange:62.5 - 8000 nM,\u003cbr\u003e\nAssay time:2h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Nitrotyrosine\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit (ab210603) is designed for the competitive measurement of Free Nitrotyrosine protein in plasma, serum, cell lysates, urine, and other sample matrices. The ELISA utilizes an Nitrotyrosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 62.5 to 8000 nM Free Nitrotyrosine, with a sensitivity of 50 nM. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.\u003cbr\u003e\nNitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NO\u003cbr\u003e\nis only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half-life in the circulation than NO\u003cbr\u003e\nlevels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present.\u003cbr\u003e\nNitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nProteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.\u003cbr\u003e\nNitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843629469865,"sku":"ab210603","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab210603","provider":"Iright","version":"1.0","type":"link"}