{"product_id":"abcam-ab210966","title":"Abcam, ab210966, Human Pro-Collagen I alpha 1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests\u003cbr\u003e\nHuman Pro-Collagen I alpha 1 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human pro-collagen I alpha 1 with a sensitivity of 5.3 pg\/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA – 450 nm readout, compatible with any standard plate reader - Available in different formats – 384-well format available for high-throughput applications - Broad sample compatibility – works with serum, plasma, cell culture extracts, tissue extracts, and cell culture supernatant - Cited in over 40 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 5.3 pg\/mL,\u003cbr\u003e\nRange:39.06 - 2500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Pro-Collagen I alpha 1 ELISA Kit ab210966 is a rapid single-wash 90-min ELISA kit to measure Human Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 5.3 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.\u003cbr\u003e\nHuman Pro-Collagen I alpha 1 ELISA Kit ab210966 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human Pro-Collagen I alpha 1\/ COL1A1 ELISA kit ab210966\u003cbr\u003e\nHuman Pro-Collagen I alpha 1\/ COL1A1 ELISA kit ab210966 has been used to study cartilage regeneration and osteoarthritis treatment\u003cbr\u003e\nand the effects of cigarette smoke and the sun on skin aging\u003cbr\u003e\nReferences:\u003cbr\u003e\nKerong Dai et al. 2022, PMID: 36114225,\u003cbr\u003e\nRoxane Pouliot et al. 2023, PMID: 37863977\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free\u003cbr\u003e\nab241825\u003cbr\u003e\n- Anti-Procollagen Type 1 antibody [EPR19126-161] - BSA and Azide free (Capture)\u003cbr\u003e\nab242540\u003cbr\u003e\n- Anti-Procollagen Type 1 antibody [EPR19126-152] - BSA and Azide free (Detector)\u003cbr\u003e\nab242788\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Pro-Collagen I alpha 1\/COL1A1 ELISA kit ab210966 is commonly used to study tissue structure and function. Human Pro-Collagen 1 alpha 1 is a component of the extracellular matrix (ECM).\u003cbr\u003e\nOther ELISA kits to measure the ECM and its role in tissue function include:\u003cbr\u003e\n- Human Fibronectin ELISA kit\u003cbr\u003e\nab219046\u003cbr\u003e\n- Human MMP1 ELISA kit\u003cbr\u003e\nab215083\u003cbr\u003e\n- Human Elastin ELISA kit\u003cbr\u003e\nab239433\u003cbr\u003e\nThe antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843535458473,"sku":"ab210966","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab210966","provider":"Iright","version":"1.0","type":"link"}