{"product_id":"abcam-ab215523","title":"Abcam, ab215523, Recombinant HIV1 Protease protein","description":"\u003cp\u003eSize: 10µg\u003cbr\u003e\nRecombinant HIV1 Protease protein is a Human immunodeficiency virus type 1 (BRU ISOLATE) Full Length protein, in the 501 to 599 aa range, expressed in Escherichia coli, with 95%, suitable for SDS-PAGE, FuncS, WB, ELISA, Dot.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:95% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, WB, ELISA, FuncS, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Reaction buffer: 20 mM Tris-HCl (pH 6.8), 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 10% Glycerol,\u003cbr\u003e\nAccession:P03367,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human immunodeficiency virus type 1 (BRU ISOLATE),\u003cbr\u003e\nStorage buffer:pH: 6 - 8.5Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.39% MES, 0.24% Tris, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhen necessary freezing (with liquid or ethanol in dry-ice) and thawing (in water at room temperature with shaking) should be done rapidly.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHIV-1 protease also called aspartyl protease is an enzyme vital for the life cycle of the HIV virus. It has a molecular mass of around 22 kilodaltons. This protease cleaves the viral polyprotein precursors generating mature protein components essential for a functional viral particle. HIV-1 protease is expressed in the cytoplasmic region of HIV-infected cells. Its unique structure with a symmetrical dimer makes it an attractive target for antiviral drugs.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHIV-1 protease functions to cleave the Gag and Gag-Pol polyprotein precursors into functional proteins. This proteolytic activity is essential in viral maturation and assembly transforming the virus into an infectious particle. HIV-1 protease does not form a complex with other proteins but operates independently to process numerous substrates within the viral lifecycle.\u003cbr\u003e\nPathways\u003cbr\u003e\nHIV-1 protease participates in the HIV replication pathway. It is important for the maturation process directly impacting the infectivity of the virus. The enzyme operates alongside other viral enzymes such as reverse transcriptase and integrase together facilitating the spread of the virus within the host. The integration of the protease activity in these pathways is necessary for the progression of the virus from a latent to an active state.\u003cbr\u003e\nHIV-1 protease plays a central role in the progression of Human Immunodeficiency Virus (HIV) and subsequent development of Acquired Immunodeficiency Syndrome (AIDS). Inhibitors targeting HIV-1 protease such as saquinavir and ritonavir act to halt the maturation of the virus and reduce viral load in patients. Connections exist between HIV-1 protease and CD4 receptor proteins which are targets in the pathogenesis of HIV. Understanding and targeting this protease remain important strategies in HIV\/AIDS treatment and management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843815461033,"sku":"ab215523","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab215523","provider":"Iright","version":"1.0","type":"link"}