{"product_id":"abcam-ab215546","title":"Abcam, ab215546, Polyethylene Glycol ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPolyethylene Glycol ELISA Kit is a competitive ELISA designed to quantify PEG (polyethylene Glycol). - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 2 - 200 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture media, Serum,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nRange:2 - 200 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPolyethylene Glycol (PEG) RabMAb\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate competitive quantitative measurement of PEGylated molecules in plasma, serum and cell culture media.\u003cbr\u003e\nThe Polyethylene Glycol (PEG) RabMAb\u003cbr\u003e\nELISA Kit operates on the basis of competition between enzyme HRP conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with anti-PEG RabMab\u003cbr\u003e\nantibody. The extent of color development resulting from interaction between HRP and the substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample. For example, the absence of PEGylated molecules in the sample will result in a bright blue color, whereas the presence of PEGylated molecules will result in decreased or no color development.\u003cbr\u003e\nThis kit is developed to determine levels of PEGylated molecules in samples such as serum, plasma or cell culture medium via ELISA.\u003cbr\u003e\nUsers must PEGylate their interested molecules. For pharmaco kinetic experiments, the PEGylated molecules will be used to construct standard curves.\u003cbr\u003e\nThe use of this kit requires the end user to have at least 250 ng of PEGylated compound of interest to use as reference standard. The included PEG-BSA is a reference sample only.\u003cbr\u003e\nPolyethylene glycol (PEG) is an O-CH\u003cbr\u003e\npolymer, which is water-soluble, nontoxic, non-antigenic, and biocompatible. Covalent conjugation of PEG to therapeutic proteins increases the\u003cbr\u003e\nin vivo\u003cbr\u003e\nstability by protecting the protein from degradation, masking its immunogenic sites and reducing clearance. Typically, PEGylation uses nonspecific reactions with nucleophilic residues and produces mixtures of PEGylated positional isomers. Qualitative and quantitative analysis of PEGylated molecules is important for both drug development and clinical application.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843568095401,"sku":"ab215546","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab215546","provider":"Iright","version":"1.0","type":"link"}