{"product_id":"abcam-ab217776","title":"Abcam, ab217776, Mouse TFPI ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse TFPI ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TFPI with a sensitivity of 1.7 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.7 pg\/mL,\u003cbr\u003e\nRange:18.75 - 1200 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse TFPI ELISA Kit (ab217776) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TFPI protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TFPI with 1.7 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nMouse TFPI (tissue factor pathway inhibitor) is a secreted protein located on the endothelial surface, within platelets, in plasma, and on monocytes. Functionally, mouse TFPI is the primary inhibitor of intravascular tissue factor (TF) activity. TF is the principal protein that initiates blood coagulation\u003cbr\u003e\nin vivo\u003cbr\u003e\n. Thus, Mouse TFPI inhibits coagulation.\u003cbr\u003e\nMouse TFPI has an acidic N-terminal region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region. The first K domain inhibits TF from binding coagulation factor VIIa. The second K domain inhibits factor Xa. The third K domain binds to heparin. Human and rat TFPI exhibit 58% and 71% amino acid identity to mouse TFPI, respectively.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843586674857,"sku":"ab217776","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab217776","provider":"Iright","version":"1.0","type":"link"}