{"product_id":"abcam-ab219824","title":"Abcam, ab219824, Anti-MTCO1 antibody [5D11-1C9] - N-terminal","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nMouse Monoclonal MTCO1 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MT-CO1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:5D11-1C9,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human MT-CO1. The exact immunogen used to generate this antibody is proprietary information.P00395\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWestern blot advice\u003cbr\u003e\nHydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification notes-Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG)., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: 0.87% Sodium chloride, 0.36% HEPES, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMTCO1 also known as COX1 or MT-CO1 is an important component of the mitochondrial respiratory chain’s complex IV commonly called cytochrome c oxidase. This target is encoded by mitochondrial DNA and contributes to the complex's catalytic core. It is a transmembrane protein with a noted molecular weight of approximately 57 kDa. MTCO1 is predominantly expressed in tissues with high energy demand such as cardiac and skeletal muscles due to their reliance on efficient oxidative phosphorylation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMTCO1 is vital for the final step of the electron transport chain catalyzing the transfer of electrons from cytochrome c to oxygen. This process facilitates the reduction of oxygen molecules to water. MTCO1 is an integral part of cytochrome c oxidase a multi-subunit enzyme complex important for cellular energy production. Proper function of MTCO1 supports ATP synthesis by maintaining electrochemical gradients across the mitochondrial inner membrane.\u003cbr\u003e\nPathways\u003cbr\u003e\nElectrons transfer through this protein is essential for effective oxidative phosphorylation and maintaining the proton gradient necessary for ATP synthesis. MTCO1 operates in tandem with proteins like COX2 within the electron transport chain to achieve optimal energy conversion and cellular respiration. The pathway interactions of MTCO1 are critical in efficiently powering cellular activities and upholding metabolic functions throughout the body.\u003cbr\u003e\nMutations or defects in MTCO1 have associations with various mitochondrial diseases such as Leber's Hereditary Optic Neuropathy (LHON) and mitochondrial complex IV deficiency. MTCO1 anomalies may disrupt normal function leading to impaired oxidative phosphorylation and energy deficits in cells. In LHON affected individuals can also demonstrate deficits linked to mutations in other mitochondrial genes like ND1 which further disrupt cellular energy balance and contribute to the clinical manifestations of these mitochondrial disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855409467561,"sku":"ab219824","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab219824","provider":"Iright","version":"1.0","type":"link"}