{"product_id":"abcam-ab219935","title":"Abcam, ab219935, Caspase-1 (active) Staining Kit - Green Fluorescence","description":"\u003cp\u003eSize: 25Test\u003cbr\u003e\nCaspase 1 (active) Staining Kit - Green Fluorescence (ab219935) is a sensitive fluorometric assay to measure caspase 1 activation in live cells.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCaspase 1 (active) Staining Kit - Green Fluorescence (ab219935) is a sensitive fluorometric assay to measure caspase 1 activation in live cells. The assay uses FAM-YVAD-FMK, which binds irreversibly to active caspase 1 in stimulated cells. The fluorescent intensity of the FAM-YVAD-FMK signal is proportional to the amount of active caspase 1 and can be easily detected at Ex\/Em = 490\/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.\u003cbr\u003e\nCaspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase-1 is primarily involved in the activation of pro-inflammatory cytokines and the process of pyropotosis. It has been proven that caspase 1 has substrate selectivity for the peptide sequence Tyr-Val-Ala-Asp (YVAD).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843622162601,"sku":"ab219935","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab219935","provider":"Iright","version":"1.0","type":"link"}