{"product_id":"abcam-ab220127","title":"Abcam, ab220127, Human Agrin Matched Antibody Pair Kit","description":"\u003cp\u003eSize: 5 x 96Tests\u003cbr\u003e\nHuman Agrin Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human Agrin by sandwich ELISA method.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nSensitivity:= 4.99 pg\/mL,\u003cbr\u003e\nRange:31.25 - 2000 pg\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Agrin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Agrin.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP\/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (\u003cbr\u003e\nab210905\u003cbr\u003e\n).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit\u003cbr\u003e\nab216945\u003cbr\u003e\n. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAgrin also known as AGRN is a large heparan sulfate proteoglycan with a molecular mass of approximately 200 kDa. This protein is mainly expressed in the neuromuscular junction and the central nervous system. Agrin has significant roles in synapse formation. It influences the clustering of acetylcholine receptors at the neuromuscular junction which is essential for communication between nerve and muscle cells. This activity is mechanically mediated through interactions with the receptor tyrosine kinase MuSK and LRP4.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Agrin protein contributes to the stabilization of synaptic structures. Its ability to induce acetylcholine receptor clustering is fundamental for the proper formation and maintenance of neuromuscular synapses. Agrin is not a lone player; it is a part of a larger signaling complex. This complex includes other proteins like MuSK which acts as a receptor playing an essential role in complex signaling pathways that regulate synaptogenesis and synaptic plasticity.\u003cbr\u003e\nPathways\u003cbr\u003e\nMany molecules associate to facilitate communication and synapse integrity via Agrin's activity. Agrin engages primarily with the MAPK\/ERK signaling pathway and the PI3K\/AKT pathway. These pathways are central for cell growth and differentiation in the central nervous system. The relationship with MuSK through these pathways highlights Agrin's importance in orchestrating the synapse assembly and stability.\u003cbr\u003e\nMutations or alterations in Agrin can lead to conditions such as congenital myasthenic syndromes and potentially impact neurodegenerative disorders like amyotrophic lateral sclerosis. In these cases its interaction with proteins like MuSK is affected leading to impaired neuromuscular transmission. Understanding the anti-Agrin antibody's function might enhance methodologies to treat these neuromuscular disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843579433129,"sku":"ab220127","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab220127","provider":"Iright","version":"1.0","type":"link"}