{"product_id":"abcam-ab220249","title":"Abcam, ab220249, Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal TMEM119 antibody. Carrier free. Suitable for ICC\/IF, Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:106-6,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse,\u003cbr\u003e\nApplications:Flow Cyt, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Human reactivity has not been tested.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab220249 is the carrier-free version of\u003cbr\u003e\nab210405\u003cbr\u003e\nPlease note that the original Bennett et al . (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author's permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.\u003cbr\u003e\nTMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46860422152361,"sku":"ab220249","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab220249","provider":"Iright","version":"1.0","type":"link"}