{"product_id":"abcam-ab221031","title":"Abcam, ab221031, Histone Extraction Kit - Rapid \/ Ultra-pure","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nab221031 Histone Extraction Kit - Rapid \/ Ultra-pure enables the extraction of core histones from mammalian cells or tissues in 1 hour using a simple procedure with 20 minutes hands-on time.\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nSample types:Tissue, Suspension cells, Adherent cells\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab221031 Histone Extraction Kit - Rapid \/ Ultra-pure enables the extraction of core histones from mammalian cells or tissues in 1 hour using a simple procedure with 20 minutes hands-on time. As post-translational modifications, such as acetylation, methylation and phosphorylation are maintained, histones extracted using this kit can be used in a wide variety of downstream applications.\u003cbr\u003e\nThis kit is compatible with three distinct protocols: Standard, Rapid and Ultra-pure Histone Extraction. The\u003cbr\u003e\nStandard Extraction\u003cbr\u003e\nprotocol is a 1 hour procedure (of which 20 minutes is hands-on) that is suitable for histone extraction from a wide range of cell lines and tissues. The\u003cbr\u003e\nRapid Extraction\u003cbr\u003e\nprotocol can be used to prepare a crude histone extract quickly for use in applications where histone purity is not critical. It is also recommended for histone extraction using fragile cell lines or in instances where extraction of cytoplasmic histones is desirable. This protocol takes 45 minutes and requires 15 minutes of hands-on time. The\u003cbr\u003e\nUltra-pure Histone Extraction\u003cbr\u003e\nprotocol is a 75-minute procedure (with 25 minutes hands-on time) that yields very pure histones from a wide range of cell lines and tissues.\u003cbr\u003e\nEach kit contains reagents to perform 100 extractions, where each extraction requires 2 x 10\u003cbr\u003e\ncells or 20 mg of tissue starting material. Typical yields are around 80 μg of histones from 2 x 10\u003cbr\u003e\nHeLa cells.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847601606825,"sku":"ab221031","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab221031","provider":"Iright","version":"1.0","type":"link"}