{"product_id":"abcam-ab222503","title":"Abcam, ab222503, Mouse IL-6 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nMouse IL-6 ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantification of mouse interleukin-6 (IL-6) with a sensitivity of 11.3 pg\/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450nm readout; works on any plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput - Broad sample compatibility - works with serum, plasma, and cell culture supernatants - Cited in over 130 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 11.3 pg\/mL,\u003cbr\u003e\nRange:15.6 - 1000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse IL-6 ELISA Kit ab222503 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 11.3 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nSimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.\u003cbr\u003e\nMouse IL-6 ELISA Kit ab222503 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Mouse IL-6 ELISA kit ab222503\u003cbr\u003e\nMouse IL-6 ELISA kit ab222503 has been used to assess inflammation in different physio-pathological contexts such as liver disease\u003cbr\u003e\n, diabetes\u003cbr\u003e\n, and aging\u003cbr\u003e\nReferences:\u003cbr\u003e\nAi-Lei Xu et al. 2024, PMID: 37902450,\u003cbr\u003e\nLili Shi et al. 2024, PMID: 38169591,\u003cbr\u003e\nYang Bao et al. 2023, PMID: 37954634\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Mouse IL-6 ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Mouse IL-6 Antibody Pair - BSA and Azide free\u003cbr\u003e\nab241663\u003cbr\u003e\n- Anti-IL-6 antibody [EPR16610-380] - BSA and Azide free (Capture)\u003cbr\u003e\nab242490\u003cbr\u003e\n- Anti-IL-6 antibody [EPR16610-318] - BSA and Azide free (Detector)\u003cbr\u003e\nab242739\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nMouse IL-6 ELISA kit ab222503 is commonly used to measure IL-6 as a marker of inflammation.\u003cbr\u003e\nOther ELISA kits to measure inflammatory markers include:\u003cbr\u003e\n- Mouse TNF alpha ELISA kit\u003cbr\u003e\nab208348\u003cbr\u003e\n- Mouse IL-10 ELISA kit\u003cbr\u003e\nab255729\u003cbr\u003e\n- Mouse IL-1 beta ELISA kit\u003cbr\u003e\nab197742\u003cbr\u003e\nIn glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.\u003cbr\u003e\nExplore our comprehensive range of glioblastoma research solutions here: https:\/\/www.abcam.com\/en-us\/products\/product-recommendations\/glioblastoma-research-tools\u003cbr\u003e\nIn Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.\u003cbr\u003e\nExplore our extensive Alzheimer's research solutions here: https:\/\/www.abcam.com\/en-us\/products\/product-recommendations\/alzheimers-disease-tools\u003cbr\u003e\nInterleukin 6 (IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843535261865,"sku":"ab222503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab222503","provider":"Iright","version":"1.0","type":"link"}