{"product_id":"abcam-ab222875","title":"Abcam, ab222875, Human FABP-1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman FABP-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FABP-1 in Human in Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026gt;= 0.66 ng\/mL,\u003cbr\u003e\nRange:1.563 - 50 ng\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman FABP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222875) is designed for the quantitative measurement of human FABP-1 protein in plasma, serum, saliva, milk, cerebral spinal fluid and cell culture samples.\u003cbr\u003e\nThe assay employs a quantitative sandwich enzyme immunoassay technique that measures human FABP-1 in approximately 4 hours. A polyclonal antibody specific for human FABP-1 has been pre-coated onto a 96-well microplate with removable strips. FABP-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human FABP-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003cbr\u003e\nMitochondrial glutamate oxaloacetate transaminase 2 (FABP-1), also called mitochondrial aspartate aminotransferase (mAspAT) and fatty acid-binding protein, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT is a pyridoxal phosphate-dependent enzyme, which exists in cytoplasmic GOT1 and inner-membrane mitochondrial FABP-1 forms. GOT plays a role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The two enzymes show close homology. FABP-1 is a dimer containing two identical subunits, each 45 kDa with about 401 amino acids. FABP-1 acetylation stimulates the malate–aspartate NADH shuttle activity and oxidative protection. It is also involved in cell proliferation and energy production.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.\u003cbr\u003e\nPathways\u003cbr\u003e\nFABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.\u003cbr\u003e\nFABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855673610409,"sku":"ab222875","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab222875","provider":"Iright","version":"1.0","type":"link"}