{"product_id":"abcam-ab223586","title":"Abcam, ab223586, Human Vitamin D Binding Protein ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Vitamin D Binding Protein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vitamin D Binding Protein with a sensitivity of 2.8 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.8 pg\/mL,\u003cbr\u003e\nRange:19.5 - 1250 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Vitamin D Binding Protein ELISA Kit ab223586 is a rapid single-wash 90-min Sandwich ELISA to measure Human Vitamin D Binding Protein in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 2.8 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Vitamin D Binding Protein SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman Vitamin D Binding Protein ELISA Kit ab223586 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-Vitamin D Binding protein antibody [EPR20695-90] - BSA and Azide free (Capture)\u003cbr\u003e\nab244921\u003cbr\u003e\n- Anti-Vitamin D Binding protein antibody [EPR20695-240] - BSA and Azide free (Detector)\u003cbr\u003e\nab244921\u003cbr\u003e\nVitamin D Binding Protein, also known as gc-globulin, is a 458 - amino acid, glycosylated protein encoded by the gene GC, which is in the albumin gene family. The primary function of this protein is to bind Vitamin D and its metabolites and to transport them to tissue. Vitamin D Binding protein is highly expressed and secreted into serum primarily from hepatic cells. There are three common alleles of the protein (Gc1f, Gc1s, and Gc2), and several minor polymorphisms. Different glycosylation sites are found on the isoforms of Vitamin D binding protein, a selectively deglycosylated form produced by B cell Я-galactosidase and T cell sialidase is known as Macrophage Activating Factor. MAF has been found to block angiogenic effects of several proteins on vascular endothelial cells.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843561509033,"sku":"ab223586","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab223586","provider":"Iright","version":"1.0","type":"link"}