{"product_id":"abcam-ab223592","title":"Abcam, ab223592, Human IL-12 p70 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests\u003cbr\u003e\nHuman IL-12 p70 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12 p70 with a sensitivity of 13.6 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma, Cell culture supernatant,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 13.6 pg\/mL,\u003cbr\u003e\nRange:31.3 - 2000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-12 p70 ELISA Kit ab223592 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-12 p70 in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 13.6 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-12 p70 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman IL-12 p70 ELISA Kit ab223592 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nIL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFN?.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843568849065,"sku":"ab223592","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab223592","provider":"Iright","version":"1.0","type":"link"}