{"product_id":"abcam-ab224852","title":"Abcam, ab224852, Recombinant Brucella abortus Malate dehydrogenase protein (His tag)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant Brucella abortus Malate dehydrogenase protein (His tag) is a Brucella abortus S19 Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with \u0026gt;90%, suitable for SDS-PAGE, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:B2S881,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Brucella abortus S19,\u003cbr\u003e\nStorage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nBrucella abortus Malate dehydrogenase also known as MDH is an enzyme that plays a significant role in the citric acid cycle. This enzyme catalyzes the conversion of malate to oxaloacetate through the reduction of NAD+ to NADH. MDH has a molecular mass of approximately 32 kDa. Within the bacterial cells it is expressed in the cytoplasm where it participates in metabolic reactions critical for energy production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme facilitates the energy transformation processes necessary for Brucella abortus to survive and replicate in host cells. It is not known to form part of a larger enzyme complex but interacts with other metabolic enzymes to ensure proper function of the tricarboxylic acid (TCA) cycle. Efficient operation of MDH is essential for providing the intermediates and reducing equivalents needed for other biological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMalate dehydrogenase contributes to the functionality of the TCA cycle and the glyoxylate shunt. These pathways are important for the survival of Brucella abortus in nutrient-limited conditions such as inside host macrophages. Malate dehydrogenase works alongside other key proteins like citrate synthase and isocitrate lyase coordinating effectively to maintain cellular energy balance and metabolic flexibility.\u003cbr\u003e\nBrucella abortus Malate dehydrogenase connects to brucellosis a zoonotic disease affecting various mammals including humans. The bacterium’s ability to proliferate within host cells partly depends on the activity of MDH and related proteins such as succinate dehydrogenase. Understanding this enzyme's role helps in devising therapeutic interventions aimed at inhibiting Brucella abortus' energy production thereby reducing infection rates and disease severity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843967799465,"sku":"ab224852","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab224852","provider":"Iright","version":"1.0","type":"link"}