{"product_id":"abcam-ab224885","title":"Abcam, ab224885, Human FMRP ELISA KIT","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman FMRP ELISA KIT is a single-wash 90-min Simplestep used to quantify Human FMRP with a sensitivity of 99.67 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 99.67 pg\/mL,\u003cbr\u003e\nRange:390.63 - 25000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman FMRP ELISA KIT ab224885 is a rapid single-wash 90-min Sandwich ELISA to measure Human FMRP in cell culture extracts, cell lysate. This SimpleStep sensitivity is 99.67 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman FMRP SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman FMRP ELISA KIT ab224885 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-FMRP antibody [EPR20008-157] - BSA and Azide free (Capture)\u003cbr\u003e\nab242905\u003cbr\u003e\n- Anti-FMRP antibody [EPR20008-144] - BSA and Azide free (Detector)\u003cbr\u003e\nab242905\u003cbr\u003e\nHuman FMRP is a 632 amino acid protein coded by the FMR1 gene. It is expressed in neurons, brain, testis, placenta and lymphocytes, epithelial tissues and at very low levels in glial cells. The most reported role for FMRP is of a regulator of translation. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression RNA-binding protein activity in intracellular RNA transport and in the regulation of translation of target mRNAs. Reports suggest it is associated with polysomes and may play a role in the transport of mRNA from the nucleus to the cytoplasm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843569111209,"sku":"ab224885","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab224885","provider":"Iright","version":"1.0","type":"link"}