{"product_id":"abcam-ab226177","title":"Abcam, ab226177, Anti-GRAF antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal GRAF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARHGAP26 aa 600-700.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human ARHGAP26 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.Q9UNA1\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab226177 was affinity purified using an epitope specific to GRAF immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate\/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGRAF also known as GAP for Rho-associated domain containing protein F has a molecular mass of approximately 110 kDa. This protein functions mechanically as a GTPase-activating protein (GAP) that regulates Rho family GTPases which are essential in controlling cell shape and movement. GRAF expression is notable in several tissues including the colon brain and heart highlighting its involvement in various physiological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGRAF plays an important role in cytoskeletal dynamics impacting cell adhesion and motility. GRAF is part of a larger signaling complex that influences cellular morphology and signal transduction pathways. This interaction underpins its engagement in cellular activities associated with structural changes and response to external stimuli. Through its activity GRAF regulates the balance of actin polymerization and depolymerization essential for maintaining cellular integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nGRAF's role intersects with the Rho signaling pathway which is critical for cell mobility and organization. This pathway contains proteins like RhoA and Rac1 where GRAF exerts its GTPase-activating function. GRAF also integrates into the integrin signaling cascade serving as a pivotal link between extracellular matrix interactions and intracellular cytoskeletal arrangements. These pathways establish GRAF's importance in coordinating cellular responses to environmental changes.\u003cbr\u003e\nGRAF's involvement has been observed in certain cancers particularly colorectal cancer and cardiovascular diseases. In colorectal cancer altered GRAF expression associates with tumor progression and metastasis partly through its interaction with dysregulated RhoA signaling. In cardiovascular conditions changes in GRAF activity influence cardiac myocyte function and may contribute to heart failure. Understanding these connections helps in appreciating GRAF's potential as a therapeutic target in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46860699467945,"sku":"ab226177","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab226177","provider":"Iright","version":"1.0","type":"link"}