{"product_id":"abcam-ab229400","title":"Abcam, ab229400, Human MIP-1 alpha\/CCL3 ELISA Kit, Fluorescent","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman MIP-1 alpha\/CCL3 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human MIP-1 alpha\/CCL3 with a sensitivity of 0.2 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Fluorescent Sandwich ELISA - 530\/570\/590 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.2 pg\/mL,\u003cbr\u003e\nRange:0.2 - 200 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMIP-1 alpha\/CCL3\u003cbr\u003e\nin vitro\u003cbr\u003e\nCatchPoint\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\n(Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MIP1a protein in human serum, plasma and cell culture supernatant.\u003cbr\u003e\nThis CatchPoint SimpleStep ELISA kit has been\u003cbr\u003e\noptimized for Molecular Devices Microplate Readers\u003cbr\u003e\n. Click\u003cbr\u003e\nfor a list of recommended Microplate Readers.\u003cbr\u003e\nIf using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at\u003cbr\u003e\nwww.softmaxpro.org\u003cbr\u003e\nThe CatchPoint\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\nemploys an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody\/analyte\/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint\u003cbr\u003e\nHRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530\/570\/590 nm Excitation\/Cutoff\/Emission.\u003cbr\u003e\n16% reactivity observed in mouse.\u003cbr\u003e\nMacrophage Inflammatory Protein 1-alpha (MIP1a, also known as CCL3) is a monokine with both inflammatory and chemokine properties. MIP-1-alpha can bind to CCR1, CCR4 and CCR5. In addition, it is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.\u003cbr\u003e\nPathways\u003cbr\u003e\nMIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.\u003cbr\u003e\nMIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843589263529,"sku":"ab229400","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab229400","provider":"Iright","version":"1.0","type":"link"}