{"product_id":"abcam-ab233486","title":"Abcam, ab233486, Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)","description":"\u003cp\u003eSize: 1 x 48Tests \/ 1 x 96Tests\u003cbr\u003e\nGlobal DNA Methylation Assay Kit (5-Methyl Cytosine) ab233486 contains all reagents necessary for the quantification of global DNA methylation. Readout on any colorimetric (450 nm) plate reader. - Detection limit as low as 0.05% methylated DNA from 100 ng of input DNA - High specific for 5-Methylcytosine with no cross-reactivity to unmethylated cytosine or hydroxymethylcytosine within the indicated concentration range of the sample DNA - Universal positive and negative controls are included for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:DNA,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nAssay time:2h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn the global DNA methylation assay ab233486, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer. The percentage of methylated DNA is proportional to the OD intensity measured.\u003cbr\u003e\nGlobal DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) protocol summary:\u003cbr\u003e\n- Bind DNA to assay wells\u003cbr\u003e\n- Wash wells, then add detection complex solution.\u003cbr\u003e\n- Wash and add color developer solution.\u003cbr\u003e\n- Measure absorbance at 450 nm\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nGlobal DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) has been used in a variety of sample type including:\u003cbr\u003e\n- Human Tumor and control peritoneal tissues 1\u003cbr\u003e\n- Human abdominal skin samples 2\u003cbr\u003e\n- Mouse brain tissues 3\u003cbr\u003e\nReferences:\u003cbr\u003e\n1 - Reamon-Buettner S et al. 2024; 2 - Ghaffarinia A et al. 2023; 3 - Spandole-Dinu S et al. 2023.\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee an alternative DNA Methylation Assay Kit for the quantification of m6A in DNA:\u003cbr\u003e\n- m6A DNA Methylation Assay Kit (Colorimetric)\u003cbr\u003e\nab233488\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843619442857,"sku":"ab233486","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab233486","provider":"Iright","version":"1.0","type":"link"}