{"product_id":"abcam-ab234044","title":"Abcam, ab234044, Griess Assay Kit \/ Griess Reagent Kit","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nGriess Assay Kit \/ Griess Reagent Kit ab234044 is a nitrite assay kit based on the Griess reaction. Includes nitrite standard for quantitation. Readout on any colorimetric (540 nm) plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Urine, Plasma, Serum, Cell Lysate,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:= 1 nmol\/well,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGriess Assay Kit \/ Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.\u003cbr\u003e\nGriess assay principle\u003cbr\u003e\nIn the Griess reaction, Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.\u003cbr\u003e\nGriess Reagent I is 1% Sulfanilamide in 5% Phosphoric Acid.\u003cbr\u003e\nGriess Reagent II is 0.1% NED (N-(1-Naphthyl)ethylenediamine dihydrochloride) in water.\u003cbr\u003e\nThe two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol\/well.\u003cbr\u003e\nGriess assay protocol summary\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\n- sequentially add Griess Reagent I, Griess Reagent II, assay buffer to wells\u003cbr\u003e\n- incubate for 10-min at room temperature\u003cbr\u003e\n- analyse on a plate reader\u003cbr\u003e\nRelated Nitrite \/ Nitrate assays\u003cbr\u003e\n- To measure total nitrate\/nitrite levels, use Nitric Oxide Assay Kit\u003cbr\u003e\nab65328\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K544 Biovision Nitrite Assay Kit (Griess Reagent). Abcam acquired Biovision in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843552333993,"sku":"ab234044","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab234044","provider":"Iright","version":"1.0","type":"link"}