{"product_id":"abcam-ab236607","title":"Abcam, ab236607, Anti-CYP8B1 antibody","description":"\u003cp\u003eSize: 100µg \/ 100µL\u003cbr\u003e\nRabbit Polyclonal CYP8B1 antibody. Suitable for IHC-P, ICC\/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYP8B1 aa 300-450.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human CYP8B1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.Q9UNU6\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity \u0026gt;95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe CYP8B1 enzyme also known as sterol 12-alpha-hydroxylase plays an important role in bile acid synthesis. It is a member of the cytochrome P450 superfamily with a mass of approximately 58 kDa. This enzyme is mainly expressed in the liver where it catalyzes the conversion of sterols to cholic acid. Through oxidation and hydroxylation reactions CYP8B1 contributes to the balance of bile acid synthesis needed for proper digestion and absorption of lipids.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe sterol 12-alpha-hydroxylase activity of CYP8B1 affects the synthesis of primary bile acids. It does not function as part of a larger protein complex but cooperates with other enzymes in the liver to ensure bile acid balance. The enzyme regulates the ratio between cholic acid and chenodeoxycholic acid influencing cholesterol homeostasis and enterohepatic circulation. Efficient bile acid synthesis by CYP8B1 affects proper lipid breakdown and nutrient absorption in the intestine.\u003cbr\u003e\nPathways\u003cbr\u003e\nCYP8B1 participates in the bile acid biosynthesis pathway specifically influencing the production of cholic acid. This process impacts the entire lipid metabolism pathway as well as cholesterol metabolism. CYP8B1's activity is complemented by other cytochrome P450 enzymes such as CYP7A1 which also play roles in converting cholesterol to bile acids. These pathways together ensure the regulated production and function of bile acids required for metabolic balance.\u003cbr\u003e\nDisturbances in CYP8B1 activity can lead to metabolic imbalances including gallbladder disease and hypercholesterolemia. Variations in the gene expression or activity of CYP8B1 can affect cholesterol levels potentially resulting in gallstones due to increased cholic acid synthesis. Moreover altered CYP8B1 function can interact with proteins involved in lipid disorders potentially linking to conditions associated with cholesterol accumulation and cardiovascular risk.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854207045801,"sku":"ab236607","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab236607","provider":"Iright","version":"1.0","type":"link"}