{"product_id":"abcam-ab238535","title":"Abcam, ab238535, DCF ROS\/RNS Assay Kit (biofluids, culture supernatant, cell lysates)","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nDCF ROS\/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (ab238535) is an assay for measuring the total free radical presence of a sample. - Complete kit to quantify total reactive oxygen species and reactive nitrogen species; including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anions. Readout on fluorometric plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nDCF ROS\/RNS Assay Kit (biofluids, culture supernatant, cell lysates)employs a proprietary quenched fluorogenic probe, dichlorodihydrofluorescin DiOxyQ (DCFH-DiOxyQ), which is a specific ROS\/RNS probe that is based on similar chemistry to the popular 2', 7'-dichlorodihydrofluorescein diacetate. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2', 7'-dichlorodihydrofluorescein (DCF). Fluorescence intensity is proportional to the total ROS\/RNS levels within the sample. The DCFH-DiOxyQ probe can react with hydrogen peroxide (H2O2), peroxyl radical (ROO·), nitric oxide (NO), and peroxynitrite anion (ONOO-).\u003cbr\u003e\nThe kit has a detection sensitivity limit of 10 pM for DCF and 40 nM for H2O2 respectively. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.\u003cbr\u003e\nDCF ROS\/RNS Assay Kit (biofluids, culture supernatant, cell lysates)protocol summary:\u003cbr\u003e\n- Unknown ROS or RNS samples or standards are added to the wells with a catalyst that helps accelerate the oxidative reaction.\u003cbr\u003e\n- After a brief incubation, the prepared DCFH probe is added to all wells and the oxidation reaction is allowed to proceed\u003cbr\u003e\n- Samples are measured fluorometrically against a hydrogen peroxide or DCF standard.\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nDCF ROS\/RNS Assay Kit (biofluids, culture supernatant, cell lysates) has been used in variety of sample type including:\u003cbr\u003e\n- Monkey kidney epithelial cells 1\u003cbr\u003e\n- Rat cardiomyocyte-derived H9c2 cells 2\u003cbr\u003e\n- Human MSCs and human fibroblasts 3\u003cbr\u003e\nReferences:\u003cbr\u003e\n1 - Velбsquez-Torres M et al. 2023; 2 - Zhao H et at 2023; 3 - Choudhury D et al. 2022.\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee alternative ROS assays available in orange (\u003cbr\u003e\nab186028\u003cbr\u003e\n), red (\u003cbr\u003e\nab186027\u003cbr\u003e\n), and deep red (\u003cbr\u003e\nab186029\u003cbr\u003e\nFor assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use\u003cbr\u003e\nab139476\u003cbr\u003e\n; to assay ROS, superoxides, and reactive nitrogen species use\u003cbr\u003e\nab139473\u003cbr\u003e\n; to assay superoxides use\u003cbr\u003e\nab219943\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843548729513,"sku":"ab238535","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab238535","provider":"Iright","version":"1.0","type":"link"}