{"product_id":"abcam-ab239711","title":"Abcam, ab239711, Cytochrome C Oxidase Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nCytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Tissue, Cell culture supernatant, Cell Lysate,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The kit provides for the fast and simple measurement of Cytochrome c oxidase activity on a 96-well plate format. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K287 Biovision Cytochrome Oxidase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.\u003cbr\u003e\nPathways\u003cbr\u003e\nComplex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.\u003cbr\u003e\nComplex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer's disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843623669929,"sku":"ab239711","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab239711","provider":"Iright","version":"1.0","type":"link"}