{"product_id":"abcam-ab239715","title":"Abcam, ab239715, Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nTotal Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit ab239715 uses an addition-only, ferrozine-based method with four 10-min incubation steps. Readout on any colorimetric (570 nm) plate reader. - quantify free iron, serum iron \/ transferrin-bound iron, and total iron-binding capacity - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Serum,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTotal Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (ab239715) provides a simple, sensitive, and high-throughput adaptable approach to detect physiological Total Iron-Binding Capacity (TIBC) and Serum Iron. Those values indicate the requisite iron for transferrin saturation and Serum Iron respectively. Those measurements can be used to detect and monitor transferrin saturation and also iron-deficiency anemia and chronic inflammatory diseases.\u003cbr\u003e\nTotal Iron-Binding Capacity (TIBC) and Serum Iron assay principles\u003cbr\u003e\nIn the combined Total Iron-Binding Capacity (TIBC) and Serum Iron assay kit, iron levels are measured using a ferrozine-based method.\u003cbr\u003e\nTo measure the levels of free iron, samples are treated with a reducing agent to convert any ferric iron (Fe3+) to form ferrous iron (Fe2+). Ferrozine then reacts with ferrous iron in the sample to form a complex measured by absorbance at 570 nm.\u003cbr\u003e\nTo measure the total level of free iron + transferrin bound iron, an acidic buffer is also used to release iron from iron carrier proteins, before the measurement of iron levels.\u003cbr\u003e\nTo measure the total iron binding capacity, an iron solution is added to saturate the iron-binding capacity of the sample. Then the same process is used to measure free iron and the total level of free iron + transferrin bound iron.\u003cbr\u003e\nSerum Iron is defined as the amount of iron in serum that is bound to transferrin. It is calculated as the total level of free iron + transferrin bound iron, less the amount of free iron.\u003cbr\u003e\nTotal Iron-Binding Capacity (TIBC) is calculated as the total level of free iron + transferrin bound iron after saturation with the iron solution, less the amount of free iron.\u003cbr\u003e\nGetting the best performance from ab239715\u003cbr\u003e\nFor optimal enzymatic activity samples should not contain EDTA or other chelating agents.\u003cbr\u003e\nThe ab239715 assay is not compatible with plasma samples collected with EDTA or citrate anticoagulants. Plasma samples collected in heparin are compatible.\u003cbr\u003e\nThis product was previously called K392 Biovision Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (Colorimetric). Abcam acquired Biovision in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843554660521,"sku":"ab239715","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab239715","provider":"Iright","version":"1.0","type":"link"}