{"product_id":"abcam-ab240182","title":"Abcam, ab240182, Anti-DOCK8 antibody [EPR12511] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal DOCK8 antibody. Carrier free. Suitable for WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12511,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240182 is the carrier-free version of\u003cbr\u003e\nab175208\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.\u003cbr\u003e\nMutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851871408297,"sku":"ab240182","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab240182","provider":"Iright","version":"1.0","type":"link"}