{"product_id":"abcam-ab240202","title":"Abcam, ab240202, Anti-LIG1 antibody [EPR12464] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal LIG1 antibody. Carrier free. Suitable for IHC-P, WB, ICC\/IF and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12464,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240202 is the carrier-free version of\u003cbr\u003e\nab177946\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe 'LIG1' protein also known as DNA ligase I plays an important role in DNA replication and repair. Mechanically LIG1 is responsible for catalyzing the formation of phosphodiester bonds between adjacent DNA strands an essential step for sealing Okazaki fragments during lagging strand synthesis. This protein features a molecular weight of approximately 102 kDa. LIG1 is predominantly expressed in the nucleus of proliferating cells with presence noted in various tissues including those undergoing rapid cell division.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDNA ligase I which is sometimes part of larger DNA replication complexes ensures the faithful duplication and repair of the genome. This protein assists with repairing single and double-strand breaks thereby maintaining genomic stability. In the context of DNA replication LIG1 is necessary for completing the joining of discontinuous segments preventing loss of genetic information. Mutations or deficiencies can compromise this repairing process potentially leading to genome instability.\u003cbr\u003e\nPathways\u003cbr\u003e\nLIG1 participates in the DNA replication pathway and the base excision repair pathway. Within these pathways LIG1 interacts with other proteins such as proliferating cell nuclear antigen (PCNA) to facilitate its recruitment to DNA and enhance its ligation efficiency. Additionally LIG1 associates with replication factor C (RFC) which acts as a clamp loader and flap endonuclease 1 (FEN1) during DNA replication and repair ensuring proper genome maintenance.\u003cbr\u003e\nDefective LIG1 function relates to genomic instability which can contribute to conditions such as immunodeficiency and increased cancer susceptibility. For example mutations in the LIG1 gene have been associated with Ligase I deficiency which presents with hypersensitivity to DNA-damaging agents and immunodeficiency. LIG1's activity is also connected to Bloom syndrome where defective DNA repair leads to chromosomal instability involving interactions with proteins such as the Bloom syndrome protein (BLM).\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851771728041,"sku":"ab240202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab240202","provider":"Iright","version":"1.0","type":"link"}