{"product_id":"abcam-ab241383","title":"Abcam, ab241383, Human Fibrinogen ELISA Kit - high sensitivity","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman Fibrinogen (FGA) ELISA kit is a single-wash, 90-minute SimpleStep ELISA ®  used to quantify fibrinogen levels with a sensitivity of 29 pg\/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric assay – 450 nm readout, compatible with any standard plate reader - Different formats for different needs – 10 x 96 well plates for bulk orders and 384-well format available for high-throughput applications - Design your own immunoassay: we also offer the conjugation-ready antibody pair in a carrier-free format\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 29 pg\/mL,\u003cbr\u003e\nRange:125 - 8000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Fibrinogen ELISA Kit - high sensitivity ab241383 is a rapid single-wash 90-min ELISA kit to measure Human Fibrinogen in saliva, urine, cell culture supernatant, milk, plasma and serum. This SimpleStep ELISA\u003cbr\u003e\nsensitivity is 29 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Fibrinogen SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant human Fibrinogen protein in serum, plasma, urine, saliva, milk, and cell culture supernatant only. Cell and tissue extract samples have not been tested with this kit.\u003cbr\u003e\nHuman Fibrinogen ELISA Kit - high sensitivity ab241383 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human Fibrinogen ELISA Kit - high sensitivity ab241383\u003cbr\u003e\nHuman Fibrinogen ELISA Kit - high sensitivity ab241383 has been used to study fibrinogen function and the effects of a novel mutation on fibinogen synthesis, secretion, and polymerization\u003cbr\u003e\n, extracorporeal bioengineered liver grafts for treating acute liver failure\u003cbr\u003e\n, and CRSPR\/Cas9 to upregulate expression of coagulation factors II, VII, IX, X, and fibrinogen\u003cbr\u003e\nReferences:\u003cbr\u003e\nFei Chen et al. 2024, PMID: 38233949,\u003cbr\u003e\nJeff J Ross et al. 2023, PMID: 37892931,\u003cbr\u003e\nMark J Osborn et al. 2022, PMID: 35563479\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human Fibrinogen ELISA Kit - high sensitivity only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity\u003cbr\u003e\nab256605\u003cbr\u003e\n- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture)\u003cbr\u003e\nab244636\u003cbr\u003e\n- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector)\u003cbr\u003e\nab244897\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Fibrinogen ELISA Kit - high sensitivity ab241383 is commonly used to study fibrinogen levels in coagulation, fibrinolysis, and liver function.\u003cbr\u003e\nOther ELISA kits to measure coagulation and liver health include:\u003cbr\u003e\n- Human AST ELISA Kit (Aspartate Aminotransferase)\u003cbr\u003e\nab263881\u003cbr\u003e\n- Human PAI1 ELISA Kit (SERPINE1)\u003cbr\u003e\nab269373\u003cbr\u003e\n- Human Plasminogen ELISA Kit (PLG)\u003cbr\u003e\nab108893\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843547254953,"sku":"ab241383","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab241383","provider":"Iright","version":"1.0","type":"link"}