{"product_id":"abcam-ab245771","title":"Abcam, ab245771, Anti-REC8 antibody [EPR16189] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal REC8 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR16189,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab245771 is the carrier-free version of\u003cbr\u003e\nab192241\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein REC8 also known as Meiotic Recombination Protein REC8 Homolog plays a mechanical role in the cohesion of sister chromatids during meiosis. This protein weighs approximately 60 kDa and is expressed mostly in germ cells during the prophase of meiosis I. REC8 is structurally related to the cohesin complex family and its primary mechanical function is to maintain the physical connection between sister chromatids ensuring accurate chromosomal segregation during meiosis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSister chromatid cohesion during meiosis depends heavily on REC8 which integrates into the cohesin protein complex. This complex consists of SMC1β SMC3 and STAG3 playing an essential role in meiotic chromosome pairing and segregation. REC8 forms part of this cohesin structure specifically ensuring precise cleavage and separation of chromatids during the anaphase I stage of meiosis which contributes critical fidelity in chromosomal inheritance.\u003cbr\u003e\nPathways\u003cbr\u003e\nREC8 influences the meiotic cell cycle and the associated recombination pathways. These pathways are highly regulated and critical for gametogenesis. Within the meiotic cell cycle pathway REC8 interacts closely with the protein RAD21 which holds key roles across various stages of the cell cycle. The well-orchestrated removal of cohesion by separase enzyme ensures successful homologous chromosome separation highlighting REC8's importance in these cellular processes.\u003cbr\u003e\nDefects in REC8 function show associations with infertility. Malfunctions in meiotic processes can lead to aneuploidies such as Down syndrome due to improper segregation of chromosomes. At the molecular level mutations or deficiencies in REC8 also connect to cohesinopathies like Cornelia de Lange syndrome where cohesion factor SMC3 could also play a part suggesting shared pathways or compensatory mechanisms related to chromosomal cohesion and segregation during meiosis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851003220137,"sku":"ab245771","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab245771","provider":"Iright","version":"1.0","type":"link"}